Signalling
Part:BBa_K176124:Design
Designed by: Danqian Liu, Zongxiao He, Hao Jiang Group: iGEM09_USTC (2009-09-22)
TetR repressible AHL->GFP Receiver (PoPS->luxR): PoPS->luxR+pLux/Tet->GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 942
Illegal BsaI.rc site found at 1686
Design Notes
Input1: PoPS
Input2: AHL
Output: GFP
Design:
Input1 PoPS generates LuxR
pLux/Tet(K176000) generates Output GFP, activated by LuxR and Input2 AHL, repressed by TetR and no aTc
Source
I0462 K176002