Signalling

Part:BBa_K176124:Design

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-09-22)


TetR repressible AHL->GFP Receiver (PoPS->luxR): PoPS->luxR+pLux/Tet->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 942
    Illegal BsaI.rc site found at 1686


Design Notes

Input1: PoPS

Input2: AHL

Output: GFP

Design:

Input1 PoPS generates LuxR

pLux/Tet(K176000) generates Output GFP, activated by LuxR and Input2 AHL, repressed by TetR and no aTc


Source

I0462 K176002

References